Related Experiment Video
Updated: Jul 13, 2025
Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
Published on: August 9, 2024
wTSA-CRAFT: an open-access web server for rapid analysis of thermal shift assay experiments
Victor Reys1, Julien Kowalewski1, Muriel Gelin1
1Centre de Biologie Structurale (CBS), CNRS UMR 5048, Université de Montpellier, INSERM U 1054, 34090 Montpellier, France.
Motivation:
The automated data processing provided by the TSA-CRAFT tool enables now to reach high throughput speed analysis of thermal shift assays. While the software is powerful and freely available, it still requires installation process and command line efforts that could be discouraging.
Results:
To simplify the procedure, we decided to make it available and easy to use by implementing it with a graphical interface via a web server, enabling a cross-platform usage from any web browsers. We developed a web server embedded version of the TSA-CRAFT tool, enabling a user-friendly graphical interface for formatting and submission of the input file and visualization of the selected thermal denaturation profiles. We describe a typical case study of buffer condition optimization of the biologically relevant APH(3')-IIb bacterial protein in a 96 deep-well thermal shift analysis screening.
Availability And Implementation:
wTSA-CRAFT is freely accessible for noncommercial usage at https://bioserv.cbs.cnrs.fr/TSA_CRAFT.
