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L-929 cells under hyperosmotic conditions: volume changes.

J S Clegg

    Journal of Cellular Physiology
    |December 1, 1986
    PubMed
    Summary
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    Mouse L-929 cells rapidly shrink in sorbitol solutions, excluding the solute. Cell volume reduction is concentration-dependent, with cells regaining volume upon return to normal conditions, revealing insights into cell dehydration.

    Area of Science:

    • Cell biology
    • Biophysics

    Background:

    • Understanding cell volume regulation is crucial for cellular homeostasis.
    • Sorbitol is a non-metabolizable solute used to alter extracellular osmolarity.

    Purpose of the Study:

    • To investigate volume changes in mouse L-929 cells upon exposure to varying sorbitol concentrations.
    • To determine the kinetics and reversibility of cell volume changes and explore underlying mechanisms.

    Main Methods:

    • Cell diameter measurements to quantify volume changes.
    • Exposure to different sorbitol concentrations.
    • Scanning electron microscopy for morphological analysis.

    Main Results:

    • Sorbitol was rapidly excluded from L-929 cells within 1 hour.

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  • Cell volume reduction was inversely proportional to sorbitol concentration up to 0.6 molal.
  • Cells maintained reduced volumes without significant regulation and rapidly recovered upon restoration of physiological conditions.
  • Cellular surface area was stored via plasma membrane convolutions and microvilli during dehydration.

    • Conclusions:

    • Mouse L-929 cells exhibit rapid, reversible volume changes in response to osmotic stress.
    • The observed surface area storage mechanism suggests adaptability to dehydration.
    • Findings provide insights into intracellular organization and cell membrane dynamics.