Progress, implications, and challenges in using humanized immune system mice in CAR-T therapy-Application evaluation...

Area of Science:

• Immunology
• Preclinical Research
• Oncology

Background:

• Humanized immune system (HIS) mice are increasingly utilized in preclinical research, outperforming traditional immunodeficient models in mimicking human immune responses and tumor microenvironments.
• Breakthroughs in tumor and other fields highlight the potential of HIS mice for evaluating novel therapies.

Purpose of the Study:

• To review and evaluate the application of HIS mice in preclinical research for chimeric antigen receptor (CAR)-T cell therapies.
• To promote the wider adoption of HIS mice by summarizing prevalent and breakthrough research in antiviral and antitumor treatments.

Main Methods:

• Selective summarization of current research on HIS mice and CAR-T cell applications.
• Exploration of HIS mice's utility in reflecting clinical patient conditions and providing detection indicators.
• Analysis of strategies to improve HIS mouse models, including reducing anti-host reactions and incorporating organoid substitution.

Main Results:

• HIS mice better simulate clinical patient conditions and tumor immune microenvironments compared to traditional immunodeficient mice.
• CAR-T cell therapy research in HIS mice shows high-efficiency detection indicators, supporting progressive research and development.
• Proposed model improvements include reducing anti-host reactions, combining models, and unifying sources for enhanced utility.

Conclusions:

• HIS mice provide a more clinically relevant platform for CAR-T cell therapy development than traditional models.
• Further development of HIS models can address limitations and expand their application in preclinical research.
• Strategies for managing relapse and toxicity in CAR-T treatment are crucial for future therapeutic advancements.