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Updated: Jul 13, 2025

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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
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Fluorescence Microscopy of Nanochannel-Confined DNA
Sriram Kk1, Fredrik Persson2, Joachim Fritzsche3
1Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden.
Methods in Molecular Biology (Clifton, N.J.)
|October 12, 2023
Abstract:
Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level, and the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments, and analyze the data.

