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Characterization of Cell Surface Glycan Profiles in Human and Mouse Corneas Using Lectin Microarrays
Rafael Martínez-Carrasco1, Pablo Argüeso1
1Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, 150 Harrison Avenue, Boston, MA 02114, USA.
Cells
|October 13, 2023
Summary
This study maps cell surface glycans in human and mouse corneas, linking glycan signatures to glycosylation gene expression. Findings reveal cell-specific glycans and interspecies differences, aiding biomarker discovery.
Area of Science:
- Glycobiology
- Ophthalmology
- Transcriptomics
Background:
- High-throughput sequencing enables single-cell glycosylation gene profiling.
- The relationship between gene signatures and glycocalyx composition is unclear.
Purpose of the Study:
- Characterize cell surface glycan composition in human and mouse corneas.
- Relate glycan composition to single-cell transcriptomic data.
- Identify cornea-specific glycan signatures and potential biomarkers.
Main Methods:
- Lectin microarrays for glycan profiling.
- Single-cell transcriptomic data analysis.
- Comparative analysis of human and mouse corneal tissues.
Main Results:
- Identified unique cell surface glycan signatures for different human corneal cell types.
- Correlated glycan signatures with glycosylation gene expression, noting O-glycan biosynthesis in epithelial cells and core fucose in stromal/endothelial cells.
- Revealed structural differences in cell surface glycan composition between human and mouse corneas.
Conclusions:
- Cell surface glycans are cell-type specific in the human cornea.
- Glycan composition correlates with glycosylation gene expression.
- Identified potential cornea-specific biomarkers and highlighted interspecies glycan differences.
