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Genome sequence of Streptomyces BM cluster phage Frankenweenie

Katherine E Cleary1, Charles Pelagalli1, Marly Cassford1

  • 1Department of Chemistry and Biochemistry, Providence College , Providence, Rhode Island, USA.

Microbiology Resource Announcements
|October 13, 2023
PubMed

Insights

A new bacteriophage, Frankenweenie, was discovered that infects Streptomyces scabiei. Its unique genome and tailspike protein may offer new ways to control this plant pathogen.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Streptomyces scabiei is a plant pathogen causing potato scab.
  • Bacteriophages are viruses that infect bacteria and can be used for biological control.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage infecting Streptomyces scabiei.
  • To analyze the genomic features of the new bacteriophage.

Main Methods:

  • Isolation and purification of bacteriophage Frankenweenie from environmental samples.
  • Whole-genome sequencing and bioinformatic analysis of the bacteriophage.
  • Comparative analysis with known bacteriophage clusters.

Main Results:

  • Frankenweenie bacteriophage was isolated and identified as infecting Streptomyces scabiei RL-34.
  • The bacteriophage possesses a 200,048-bp genome with 366 predicted genes.
  • Frankenweenie belongs to cluster BM and exhibits a unique tailspike protein.

Conclusions:

  • Frankenweenie represents a novel bacteriophage with potential applications in controlling Streptomyces scabiei.
  • The unique tailspike protein may confer a broader host range, warranting further investigation.
Keywords:
Streptomycesgenome analysisphage

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