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Development-dependent replication of minute virus of mice in differentiated mouse testicular cell lines
Abstract:
The replication of the autonomous parvovirus, minute virus of mice (MVM), requires mitotically active cells and depends on certain factors expressed by cells of particular differentiated phenotype. As an approach to the understanding of these helper functions, we studied the interaction of the fibrotropic [MVM(p)] and the lymphotropic [MVM(i)] strains of MVM with two differentiated cell lines from mouse testicular epithelial origins. The relative support given to viral expression by these cell lines varied extensively. Cells from Sertoli origin (TM4) were permissive to MVM(p) but were mostly restrictive to MVM(i). The other cell line, of Leydig cell origin (TM3), was highly restrictive to both viral strains, but the blocks to their growth in these cells were localized at different stages of their growth cycle, suggesting that the replication of MVM in these cells requires tissue-specific helper functions during at least two stages of viral replication.
Insights
Minute virus of mice (MVM) replication needs specific cellular factors. Mouse testicular cells show differential support for MVM strains, indicating tissue-specific helper functions are crucial for viral replication.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Autonomous parvoviruses like minute virus of mice (MVM) require host cell factors for replication.
- Cellular differentiation influences the permissiveness to MVM infection.
Purpose of the Study:
- To investigate the role of tissue-specific helper functions in MVM replication.
- To understand the differential interaction of MVM strains with differentiated mouse testicular cell lines.
Main Methods:
- Utilized two differentiated mouse testicular epithelial cell lines (Sertoli-derived TM4 and Leydig-derived TM3).
- Assessed the replication and expression of fibrotropic [MVM(p)] and lymphotropic [MVM(i)] MVM strains in these cell lines.
Main Results:
- Sertoli cells (TM4) supported MVM(p) but restricted MVM(i).
- Leydig cells (TM3) restricted both MVM strains, with distinct blocks at different replication stages.
- Replication of MVM in TM3 cells suggests requirement for helper functions at multiple stages.
Conclusions:
- MVM replication is dependent on tissue-specific cellular helper functions.
- Differential permissiveness of testicular cell lines highlights stage-specific requirements for MVM growth.