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Updated: Jul 13, 2025

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
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Quantitative phase imaging based on motionless optical scanning holography.

Naru Yoneda, Osamu Matoba, Yusuke Saita

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    This summary is machine-generated.

    Motionless optical scanning holography (MOSH) now enables quantitative phase imaging (QPI), overcoming previous limitations. This advancement, MOSH-based QPI (MOSH-QPI), simplifies setups for 3D imaging applications.

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    Area of Science:

    • Optical physics
    • Holography
    • 3D imaging

    Background:

    • Optical scanning holography (OSH) offers 3D fluorescent imaging capabilities.
    • Traditional OSH setups are complex, requiring phase shifters, scanners, and interferometers.
    • Existing motionless optical scanning holography (MOSH) lacks quantitative phase imaging (QPI) due to incoherent holograms.

    Purpose of the Study:

    • To propose and demonstrate MOSH-based QPI (MOSH-QPI).
    • To simplify optical scanning holography for quantitative phase imaging.
    • To expand the applications of MOSH through QPI.

    Main Methods:

    • Development of MOSH-QPI by adapting MOSH for quantitative phase information.
    • Implementation of a spatially divided phase-shifting technique to minimize measurements.
    • Experimental validation using a microlens array and comparison with conventional Mach-Zehnder interferometry.

    Main Results:

    • Successful demonstration of MOSH-QPI, enabling quantitative phase measurements.
    • Validation of MOSH-QPI feasibility through phase distribution measurement of a microlens array.
    • Comparison showing comparable results to conventional interferometric methods for practical samples.

    Conclusions:

    • MOSH-QPI is a feasible technique for quantitative phase imaging.
    • The proposed method simplifies optical setups compared to traditional OSH.
    • MOSH-QPI opens new avenues for various imaging applications requiring phase information.