Molecularly imprinted nanoparticles doped graphene oxide based electrochemical platform for highly sensitive and...

A highly sensitive and selective electrochemical sensor was developed using a surface modified glassy carbon electrode (GCE) through molecularly imprinted polymerization on the surface of vinyltrimethoxysilane (VTMS) coated magnetic nanoparticle (Fe₃O₄) decorated silver nanoparticles incorporated graphene oxide, GO (VTMS-Fe₃O₄/AgGO) for L- Tyrosine (Tyr) detection. A molecular imprinting technique based on free radical polymerization was applied to synthesize molecularly imprinted Methacrylic acid (MAA) and Acrylamide (AA) grafted VTMS-Fe₃O₄/AgGO polymer (MAA/AA-g- VTMS-Fe₃O₄/AgGO) designated as MIP and non-imprinted polymer (NIP). The structure and morphology of the prepared polymers were FTIR, XRD, FE-SEM and VSM. MIP and NIP were chosen for modifying the GCE surface by drop casting process to construct the sensors and their electrochemical properties were characterized via EIS and CV. Compared with NIP/GCE sensor, MIP /GCE sensor exhibits excellent sensing response towards Tyr with a wide linear range of 0.25 × 10^-13 M to 0.10 × 10^-3 M and the limit of detection and limit of quantification as 0.15 × 10^-13 M and 0.50 × 10^-13 M, respectively with R² value of 0.9934 by DPV technique. Moreover, MIP/GCE sensor exhibits long-time storage, excellent selectivity and good stability in multiple cycle usage. The practical applicability of MIP/GCE sensor was tested in human blood serum sample. The recovery percentage was obtained between 98.8% and 106.0% with a relative standard deviation (RSD) between 1.01% and 1.59%. Results of the investigations revealed the clinical applicability of the MIP/GCE sensor.