Related Experiment Video
Updated: Jul 13, 2025
08:00
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
4.4K
Dynamic mRNA expression during chicken ovarian follicle development.
Hua Kui1,2, Penghao Li2, Tao Wang1
1School of Pharmacy, Chengdu University, Chengdu 610106, People's Republic of China.
G3 (Bethesda, Md.)
|October 13, 2023
Summary
This study maps gene expression during chicken ovarian follicle development, identifying key stages. Findings advance understanding of poultry egg production and ovarian cancer risk factors.
Area of Science:
- Reproductive Biology
- Genomics
- Poultry Science
Background:
- Ovarian follicle development is crucial for poultry production and understanding ovarian cancer.
- Domestic hens offer a model for studying ovarian cancer risk factors.
Purpose of the Study:
- To provide high-quality RNA sequencing data for chicken follicular granulosa cells across 10 developmental stages.
- To analyze gene expression patterns and identify key transcriptional changes during follicular development.
Main Methods:
- RNA sequencing of chicken follicular granulosa cells from 10 developmental stages.
- Gene expression, time-series, and functional enrichment analyses.
- Identification of representative stages: small white follicle (SWF), F1 hierarchical follicles (F1), and postovulatory follicles (POFs).
Main Results:
- Generated over 200 Gb of clean RNA sequencing data.
- SWF, F1, and POFs represent distinct transcriptional profiles of prehierarchical, preovulatory, and postovulatory stages.
- The F1 stage exhibited the most significant gene expression divergence from the POF stage.
Conclusions:
- This study provides a foundational dataset for understanding chicken egg-laying performance.
- The findings contribute to elucidating molecular mechanisms relevant to human ovarian disease.
Related Concept Videos
01:20Folliculogenesis
832
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
832
01:22Oogenesis
1.6K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
1.6K
01:23Regulation of Expression at Multiple Steps
936
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
936
02:24Regulation of Expression Occurs at Multiple Steps
22.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.7K