Physiological response of thiamethoxam and ulexite in rainbow trout: A neural network-mediated approach

Fish, which are in constant contact with water, serve as an important ecological indicator of aquatic environment health. Therefore, in this study, in the name of neural degeneration, thiamethoxam (TMX) insecticide in the cerebral tissue of Oncorhynchus mykiss; neurotoxic endpoints such as biomarkers of oxidative stress, DNA damage and the status of antioxidant enzymes have been identified. Antioxidant enzyme (CAT, SOD, GPx, GSH) activities were significantly inhibited by TMX administration, and MDA and MPO values increased as a result of the stimulation of ROS (p < 0.05). It was interpreted that ulexite (UX) added to the medium was effective in favor of antioxidants and tried to prevent MDA and MPO levels. It was determined that Nrf-2, one of the inflammation parameters, was inhibited as a result of TMX application, and the supplementation of UX to the medium created merits similar to the no treatment group. In the 48th and 96th hour analyses of cerebral tissue, it was determined that IL-6 and TNF-α values were induced in TMX applied groups and UX tried to inhibit this situation. It was commented that TMX induced DNA damage and apoptosis at 48th-96th h, whereas UX suppressed this situation. The results provide possible in vivo evidence that UX supplements can reduce TMX-mediated oxidative stress and brain damage in O. mykiss brain tissue.