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DSE inhibits melanoma progression by regulating tumor immune cell infiltration and VCAN

Lin Xia1, Maoxiao Feng2, Yidan Ren3

  • 1Department of Plastic Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Cell Death Discovery
|October 13, 2023
PubMed

Insights

Dermatan sulfate epimerase (DSE) is downregulated in melanoma. High DSE expression inhibits melanoma cell growth and invasion, potentially by increasing immune cell infiltration and VCAN expression.

Area of Science:

  • Biochemistry
  • Oncology
  • Immunology

Background:

  • Dermatan sulfate epimerase (DSE) modifies chondroitin sulfate and influences growth factor signaling in cancer.
  • DSE's role in human melanoma progression and its underlying mechanisms remain uninvestigated.

Purpose of the Study:

  • To investigate the expression and function of DSE in human melanoma.
  • To elucidate the mechanisms by which DSE influences melanoma progression.

Main Methods:

  • Proteomic analysis of melanoma tissues.
  • Construction of DSE-silenced and overexpressed melanoma cell lines.
  • Flow cytometry to analyze immune cell subpopulations.
  • VCAN expression analysis.

Main Results:

  • DSE was significantly downregulated in melanoma tissues.
  • DSE overexpression inhibited melanoma cell proliferation, migration, and invasion.
  • High DSE expression correlated with increased killer immune cell infiltration.
  • DSE promoted VCAN expression, which inhibited melanoma cell activity.

Conclusions:

  • DSE is downregulated in melanoma, and its high expression acts as a tumor suppressor.
  • DSE may promote melanoma progression by enhancing immune cell infiltration and VCAN expression.

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