Evaluating Prophylactic Effect of Bovine Colostrum on Intestinal Barrier Function in Zonulin Transgenic Mice: A...

Area of Science:

• Gastroenterology and Immunology
• Nutritional Science
• Molecular Biology

Background:

• Intestinal barrier disruption increases chronic inflammation risk.
• Dietary components may influence gut barrier function.
• Bovine colostrum (BC) possesses gut-barrier fortifying properties.

Purpose of the Study:

• Investigate the molecular mechanisms of BC's effect on the intestinal barrier.
• Analyze the transcriptome of BC-treated Zonulin transgenic mice (Ztm).
• Identify key genes and pathways influenced by BC treatment.

Main Methods:

• RNA sequencing of duodenum, colon, and brain tissues from Ztm and wild-type mice.
• Analysis of differentially expressed genes (DEGs) and transcripts (DETs).
• Bioinformatic examination of molecular pathways and interactions.

Main Results:

• BC treatment altered gene expression in Ztm mice, affecting tight junction components like cingulin (Cgn) and claudin 12 (Cldn12).
• Sex-specific differences observed: females upregulated Cgn/Cldn12, males upregulated occludin (Ocln) and Arhgf2.
• BC influenced tight junction and NF-kB signaling pathways.

Conclusions:

• BC treatment modulates gene expression related to intestinal barrier integrity in Ztm mice.
• Identified hub genes and pathways impacted by BC, offering insights into gut health.
• Findings suggest potential for BC in dietary interventions for gut barrier enhancement.