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Continuous Cropping Inhibits Photosynthesis of Polygonatum odoratum
Yan Wang1,2, Yunyun Zhou1,3, Jing Ye1
1College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China.
Plants (Basel, Switzerland)
|October 14, 2023
Summary
Continuous cropping of Polygonatum odoratum suppresses photosynthesis and carbon assimilation. This study reveals key gene expression changes impacting yield and quality, offering insights for improving crop management.
Area of Science:
- Plant Physiology
- Molecular Biology
- Agricultural Science
Background:
- Polygonatum odoratum (Mill.) Druce, a plant with significant medicinal value, suffers from yield and quality decline due to continuous cropping (CC) and consecutive monoculture problems (CMP).
- Photosynthesis is crucial for plant growth, and enhancing it is a key strategy for increasing crop yields.
- Understanding the impact of CC on P. odoratum's photosynthetic mechanisms is vital for sustainable cultivation.
Purpose of the Study:
- To investigate the effects of continuous cropping on the photosynthetic physiology and gene expression in Polygonatum odoratum leaves.
- To identify molecular mechanisms underlying the decline in yield and quality associated with consecutive monoculture.
Main Methods:
- Analysis of physiochemical indexes including chlorophyll content and light response curves (LRCs).
- Measurement of photosynthetic parameters such as Fv/Fm, Fv/F0, Fm/F0, Piabs, ABS/RC, TRo/RC, ETo/RC, and DIo/RC.
- RNA-sequencing (RNA-seq) to identify differentially expressed genes (DEGs) between continuously cropped and first-cropped plants.
Main Results:
- Continuous cropping significantly altered physiochemical indexes and photosynthetic parameters in P. odoratum leaves.
- RNA-seq identified 13,798 differentially expressed genes (DEGs), with 7932 upregulated and 5860 downregulated.
- Genes involved in photosynthesis and carbon assimilation, including components of photosystems I and II, the cytochrome b6/f complex, and the Calvin cycle, showed significantly decreased expression under continuous cropping.
Conclusions:
- Continuous cropping suppresses photosynthesis and carbon assimilation mechanisms in Polygonatum odoratum.
- The study identifies specific molecular pathways affected by consecutive monoculture, providing targets for improving photosynthetic efficiency.
- Findings offer potential strategies to mitigate the negative impacts of continuous cropping on P. odoratum cultivation.
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