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Activated oxygen molecules generated by electrical stimulation affect vascular smooth muscle.

B Greenberg, K Rhoden, P J Barnes

    Journal of Molecular and Cellular Cardiology
    |September 1, 1986
    PubMed
    Summary

    Electrical field stimulation of solutions generates activated oxygen molecules, like hydrogen peroxide, which can relax blood vessels. These effects are important to consider during vascular research.

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    Area of Science:

    • Physiology
    • Biochemistry
    • Vascular Biology

    Background:

    • Electrical field stimulation (EFS) is used to study neural control of blood vessels.
    • EFS can generate reactive oxygen species (ROS), potentially confounding experimental results.
    • The specific ROS and their vascular effects from EFS require clarification.

    Purpose of the Study:

    • To investigate the generation of activated oxygen molecules by EFS in a physiological buffer.
    • To determine the direct effects of EFS-generated molecules on vascular smooth muscle.
    • To elucidate the role of specific ROS, such as hydrogen peroxide, in EFS-induced vascular responses.

    Main Methods:

    • Aerated Krebs-Henseleit solution was subjected to electrical field stimulation.
    • The stimulated solution was applied to pre-contracted bovine pulmonary artery segments.

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  • Vessel relaxation was measured, and the involvement of ROS was assessed using scavengers like ascorbate, catalase, and superoxide dismutase.

    • Main Results:

    • EFS of the buffer solution induced significant vascular relaxation.
    • The relaxation was concentration-dependent on EFS parameters (voltage, frequency, pulse duration).
    • Hydrogen peroxide was identified as the key ROS mediating relaxation, as indicated by inhibition with catalase and ascorbate.

    Conclusions:

    • Low-intensity EFS in oxygenated buffer solutions generates vasoactive ROS, primarily hydrogen peroxide.
    • These ROS can directly relax vascular smooth muscle and modulate responses to vasoactive agents.
    • The generation of ROS by EFS must be accounted for when interpreting studies on vascular function and pharmacology.