Solution-State 2D NMR Spectroscopy of Mixtures HyperpolarizedUsing Optically Polarized Crystals

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Physical Chemistry

Background:

• Hyperpolarized NOE System (HYPNOESYS) enhances solution-state NMR sensitivity using optically polarized crystals.
• Traditional HYPNOESYS is a single-shot technique, limiting its application in multidimensional NMR.
• There is a need for more information-rich and sensitive NMR methods compatible with multidimensional analysis.

Purpose of the Study:

• To adapt the HYPNOESYS method for obtaining 2D NMR spectra.
• To demonstrate the feasibility of single-scan acquisition for HYPNOESYS-based 2D NMR.
• To establish a sensitive, information-rich, and affordable analytical method.

Main Methods:

• Utilized optically polarized crystals for hyperpolarization.
• Developed single-scan acquisition protocols for 2D NMR experiments.
• Employed a benchtop NMR spectrometer for analysis.

Main Results:

• Successfully acquired 2D NMR spectra from HYPNOESYS-polarized samples.
• Demonstrated the method's effectiveness with a mixture of terpene molecules.
• Achieved enhanced sensitivity in multidimensional NMR analysis.

Conclusions:

• The adapted HYPNOESYS method enables 2D NMR spectroscopy.
• This approach offers a sensitive, information-rich, and affordable analytical solution.
• The technique is compatible with benchtop NMR spectrometers, increasing accessibility.