Effect of pachymaran on oxidative stress and DNA damage induced by formaldehyde
Zhijun Zhang1, Yuan Yang1, Changjun Hu2
1College of Public Health and Laboratory Medicine, Hunan University of Medicine, 492 Jinxi South Road, Huaihua, Hunan, 418000, People's Republic of China.
Abstract:
To further explore the pharmacological effect of pachymaran, this article studied the inhibition of pachymaran on oxidative stress and genetic damage induced by formaldehyde. 40 adult Kunming male mice were randomly divided into four groups with different interventions. One week later, the contents of serum SOD, GR, MDA, DNA-protein crosslink (DPC), 8-hydroxydeoxyguanosine (8-OHDG) and DNA adduct were determined by ELISA. The results showed that there were statistically significant differences in the contents of SOD, GR and MDA among the four groups (P < 0.01). The activity of SOD and GR increased along with the increase of pachymaran dosage (SOD: rs = 0.912, P < 0.01; GR: rs = 0.857, P < 0.01), while the content of MDA showing a significant negative correlation (rs = - 0.893, P < 0.01). There were statistically significant differences in the levels of DPC, 8-OHDG and DNA adduct among the four groups (DPC and DNA adduct: P < 0.01, 8-OHDG: P < 0.05), the concentration decreased along with the increase of pachymaran dosage (DPC: rs = - 0.855, P < 0.01; 8-OHDG:rs = - 0.412, P < 0.05, DNA adduct: γs = - 0.869, P < 0.01). It can be inferred that pachymaran can inhibit oxidative stress and DNA damage induced by formaldehyde with the dose-effect relationship.
Insights
Pachymaran effectively inhibits formaldehyde-induced oxidative stress and genetic damage in mice. This study demonstrates that pachymaran increases antioxidant enzyme activity (superoxide dismutase and glutathione reductase) and reduces lipid peroxidation (malondialdehyde) and DNA damage markers in a dose-dependent manner.
Area of Science:
- Pharmacology
- Toxicology
- Biochemistry
Background:
- Formaldehyde is a known inducer of oxidative stress and genetic damage.
- Pachymaran is a compound with potential pharmacological effects that warrant investigation.
- Understanding the protective mechanisms of natural compounds against toxic insults is crucial for developing therapeutic strategies.
Purpose of the Study:
- To investigate the inhibitory effects of pachymaran on formaldehyde-induced oxidative stress.
- To evaluate the protective role of pachymaran against formaldehyde-induced genetic damage.
- To establish a dose-effect relationship for pachymaran's protective actions.
Main Methods:
- Adult male Kunming mice were randomly assigned to four experimental groups with varying interventions.
- Mice were treated for one week, followed by serum analysis.
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify serum levels of superoxide dismutase (SOD), glutathione reductase (GR), malondialdehyde (MDA), DNA-protein crosslink (DPC), 8-hydroxydeoxyguanosine (8-OHDG), and DNA adducts.
Main Results:
- Pachymaran administration significantly altered serum levels of SOD, GR, and MDA (P < 0.01).
- SOD and GR activities increased, while MDA levels decreased with increasing pachymaran dosage, indicating reduced oxidative stress.
- Pachymaran treatment dose-dependently reduced levels of DPC, 8-OHDG, and DNA adducts (P < 0.01 for DPC and DNA adducts; P < 0.05 for 8-OHDG), signifying protection against genetic damage.
Conclusions:
- Pachymaran exhibits significant inhibitory effects against formaldehyde-induced oxidative stress.
- Pachymaran demonstrates a protective role in mitigating formaldehyde-induced DNA damage.
- A clear dose-effect relationship exists for pachymaran's protective actions against formaldehyde toxicity.
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