Electrochemical techniques for label-free and early detection of growing microbial cells and biofilms

Area of Science:

• Microbiology and Electrochemistry
• Biosensing Technologies

Background:

• Antimicrobial resistance (AMR) is a significant global health threat driven by antimicrobial misuse.
• Microbial biofilms exhibit increased resistance and virulence compared to planktonic forms, complicating treatment.
• Early detection of planktonic microbes and biofilms is crucial for effective treatment and AMR containment.

Approach:

• This review explores electrochemical techniques, specifically impedimetric and voltamperometric methods.
• Highlights recent advancements in electrochemical biosensing for microbial detection.
• Illustrates applications in antibiotic susceptibility testing and biofilm monitoring.

Key Points:

• Electrochemical methods enable sensitive and rapid detection of microbial growth stages.
• Impedimetric and voltamperometric techniques show promise for point-of-need microbial diagnostics.
• These methods can differentiate between planktonic and biofilm microbial states.

Conclusions:

• Electrochemical biosensors provide a viable alternative to traditional methods for microbial detection.
• Advancements in electrochemical techniques support the development of novel strategies against antimicrobial resistance.
• Early detection via electrochemical sensing is key to optimizing antimicrobial use and patient outcomes.