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Detection of Lotmaria passim in honeybees from Emilia Romagna (Italy) based on a culture method
Cecilia Rudelli1, Gloria Isani1, Giulia Andreani1
1Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, via Tolara di sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Abstract:
Lotmaria passim is considered an emerging field of study in honeybee pathology, since it can threaten the health of the colony leading to a higher mortality rate. However, there is a lack of knowledge regarding the diffusion of this trypanosomatid in Italy. In this study, we highlight the presence of L. passim in the province of Bologna through its culture isolation from honeybee guts and microscopic observation.
Insights
Lotmaria passim, a honeybee pathogen, was found in Bologna, Italy. This study isolated the trypanosomatid from honeybee guts, highlighting its presence and potential threat to colony health.
Area of Science:
- Veterinary Pathology
- Apiculture Science
- Microbiology
Background:
- Lotmaria passim is an emerging pathogen impacting honeybee colony health and survival.
- Limited data exists on the geographical distribution of L. passim within Italy.
- Understanding L. passim diffusion is crucial for effective honeybee disease management.
Purpose of the Study:
- To investigate the presence and diffusion of Lotmaria passim in Italy.
- To identify L. passim in honeybee populations within the Bologna province.
- To contribute to the knowledge of honeybee pathology in the region.
Main Methods:
- Isolation of Lotmaria passim through microbial culture techniques.
- Microscopic examination for pathogen identification.
- Sampling of honeybee gut contents for analysis.
Main Results:
- Successful isolation and identification of L. passim from honeybee gut samples.
- Confirmation of L. passim presence in the province of Bologna.
- Demonstration of L. passim as a viable pathogen in Italian honeybee populations.
Conclusions:
- Lotmaria passim is present in the honeybee population of Bologna, Italy.
- The findings underscore the need for further research on L. passim epidemiology in Italy.
- This study provides baseline data for monitoring and controlling L. passim in Italian apiaries.
