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Related Experiment Video

Updated: Jul 13, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

An Automated Nanowell-Array Workflow for Quantitative Multiplexed Single-Cell Proteomics Sample Preparation at High

Claudia Ctortecka¹, David Hartlmayr², Anjali Seth³

¹Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Molecular & Cellular Proteomics : MCP

|October 15, 2023

Summary

This summary is machine-generated.

The proteoCHIP streamlines single-cell proteomics sample preparation, enabling high-throughput, multiplexed analysis. This automated workflow enhances sensitivity and protein identification, facilitating biological discovery in single-cell studies.

Keywords:

automated sample preparation high field asymmetric waveform ion mobility spectrometry isobaric peptide labeling proteoCHIP single-cell proteomics

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Last Updated: Jul 13, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Area of Science:

Proteomics
Cell Biology
Analytical Chemistry

Background:

Single-cell resolution reveals cellular heterogeneity in previously assumed homogenous populations.
Advances in mass spectrometry instrumentation contrast with limitations in efficient single-cell sample preparation.

Purpose of the Study:

To introduce the proteoCHIP, a universal, automated sample preparation method for multiplexed single-cell proteomics.
To enhance sensitivity, throughput, and protein identification in single-cell proteomic analyses.

Main Methods:

Automated single-cell isolation and picoliter dispensing using the cellenONE system.
Multiplexed labeling up to 16-plex (TMT10-plex) with the proteoCHIP.
Mass spectrometry acquisition strategies including wide precursor isolation windows (2 Da).

Main Results:

Achieved high sensitivity and throughput for single-cell proteomics with the proteoCHIP.
Identified approximately 1500 protein groups per TMT10-plex, with reduced need for carrier proteome.
Identified an average of nearly 2000 proteins per TMT10-plex across 170 multiplexed single cells, distinguishing human cell types.

Conclusions:

The proteoCHIP offers a versatile, automated, and sensitive solution for single-cell proteomics sample preparation.
This workflow integrates efficient sample prep, advanced MS techniques, and data analysis for robust single-cell proteomic studies.
The proteoCHIP approach is poised to advance biological applications of single-cell proteomics in research laboratories.