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Updated: Jul 13, 2025
Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice
Published on: May 13, 2019
A surgical mouse model of neonatal right ventricular outflow tract obstruction by pulmonary artery banding
Debao Li1, Haifa Hong2, Minghui Li3
1Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Department of Pediatric Surgery, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.
Backgroud:
Diseased animal models play an extremely important role in preclinical research. Lacking the corresponding animal models, many basic research studies cannot be carried out, and the conclusions obtained are incomplete or even incorrect. Right ventricular (RV) outflow tract (RVOT) obstruction leads to RV pressure overload (PO) and reduced pulmonary blood flow (RPF), which are 2 of the most important pathophysiological characteristics in pediatric cardiovascular diseases and seriously affect the survival rate and long-term quality of life of many children. Due to the lack of a neonatal mouse model for RVOT obstruction, it is largely unknown how RV PO and RPF regulate postnatal RV and pulmonary development. The aim of this study was to construct a neonatal RVOT obstruction mouse model.
Methods And Results:
Here, we first introduced a neonatal mouse model of RVOT obstruction by pulmonary artery banding (PAB) on postnatal day 1. PAB induced neonatal RVOT obstruction, RV PO, and RPF. Neonatal RV PO induced cardiomyocyte proliferation, and neonatal RPF induced pulmonary dysplasia, the 2 features that are not observed in adult RVOT obstruction. As a result, PAB neonates exhibited overall developmental dysplasia, a sign similar to that of children with RVOT obstruction.
Conclusions:
Because many pediatric cardiovascular diseases are associated with RV PO and RPF, the introduction of a neonatal mouse model of RVOT obstruction may greatly enhance our understanding of these diseases and eventually improve or save the lives of many children.
Insights
Researchers developed a neonatal mouse model for right ventricular outflow tract (RVOT) obstruction. This model mimics pediatric cardiovascular disease, enabling study of how RV outflow tract obstruction impacts development.
Area of Science:
- Cardiovascular Research
- Developmental Biology
- Animal Models
Background:
- Right ventricular outflow tract (RVOT) obstruction causes critical issues in pediatric cardiovascular diseases, including pressure overload (PO) and reduced pulmonary blood flow (RPF).
- Current research is limited by the absence of a neonatal mouse model to study RVOT obstruction's effects on postnatal development.
- Understanding these effects is crucial for improving treatment and outcomes in affected children.
Purpose of the Study:
- To establish a neonatal mouse model of RVOT obstruction.
- To investigate the impact of neonatal RVOT obstruction on right ventricular (RV) and pulmonary development.
- To provide a preclinical tool for studying pediatric cardiovascular diseases characterized by RV PO and RPF.
Main Methods:
- A neonatal mouse model was created using pulmonary artery banding (PAB) on postnatal day 1.
- The model successfully induced RVOT obstruction, RV pressure overload (PO), and reduced pulmonary blood flow (RPF) in neonates.
- Observed developmental changes were compared to those in adult models and human pediatric conditions.
Main Results:
- Pulmonary artery banding (PAB) in neonates resulted in RVOT obstruction, RV PO, and RPF.
- Neonatal RV PO stimulated cardiomyocyte proliferation, while RPF led to pulmonary dysplasia.
- The PAB neonates displayed developmental dysplasia, mirroring conditions seen in children with RVOT obstruction.
Conclusions:
- The developed neonatal RVOT obstruction mouse model accurately reflects key pathophysiological features of pediatric cardiovascular diseases.
- This model allows for the study of how neonatal RV PO and RPF influence RV and pulmonary development.
- The model holds significant potential for advancing research into pediatric cardiovascular diseases and improving patient outcomes.
