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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Approaches and challenges in genome-wide circular RNA identification and quantification
Xu-Kai Ma1, Si-Nan Zhai2, Li Yang1
1Center for Molecular Medicine, Children's Hospital, Fudan University and Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
Trends in Genetics : TIG
|October 15, 2023
Summary
Circular RNAs (circRNAs), generated from exon back-splicing, are increasingly studied for their regulatory roles. This review overviews methods for their genome-wide identification, quantification, and characterization.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Circular RNAs (circRNAs) are formed by back-splicing of exons and are found across species.
- While often low-abundance, some circRNAs play significant regulatory roles in biological processes.
- Profiling circRNAs is essential for understanding their functions.
Purpose of the Study:
- To provide an overview of current strategies for genome-wide circRNA identification.
- To discuss methods for accurate circRNA quantification and characterization.
- To highlight the scope and limitations of existing techniques.
Main Methods:
- Review of transcriptome enrichment strategies.
- Analysis of deep sequencing approaches for circRNA detection.
- Evaluation of computational tools for circRNA identification and quantification.
Main Results:
- Various transcriptome enrichment and bioinformatic tools exist for circRNA detection.
- Different strategies have varying scopes and limitations.
- Accurate quantification and characterization require careful selection of methods.
Conclusions:
- Understanding the strengths and weaknesses of different circRNA identification and quantification strategies is crucial.
- This knowledge facilitates the annotation and characterization of functional circRNAs.
- Further refinement of methods will enhance circRNA research.
