Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

2.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
2.1K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

425
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
425

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRPV1 Agonist Capsaicin Enhances Oxidative-Stress Resistance and Regeneration in Dorsal Root Ganglia and Schwann Cells.

Cells·2026
Same author

Light-induced quantum friction of carbon nanotubes in water.

Nature·2026
Same author

Packed by the Surface: Relating Surface Structure and Solvation Properties at Solid/Water Interfaces.

Journal of chemical theory and computation·2026
Same author

Kinetics and Spatial Distribution of β-Sheet Development in TDP-43<sub>CTD</sub> Condensate Maturation.

ACS chemical neuroscience·2026
Same author

The effect of ions on water alignment and its nonlinear optical response at metal electrodes.

Faraday discussions·2026
Same author

A Novel 3D Semi-Automated Full Quantification Technique for Detection of Intraneural Phospho-α-Synuclein in Skin Biopsies.

European journal of neurology·2026

Related Experiment Video

Updated: Jul 13, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K

Hydration makes a difference! How to tune protein complexes between liquid-liquid and liquid-solid phase separation.

Sashary Ramos1, Janine Kamps2, Simone Pezzotti1

  • 1Department of Physical Chemistry II, Ruhr University Bochum, Bochum, Germany. martina.havenith@rub.de.

Physical Chemistry Chemical Physics : PCCP
|October 16, 2023
PubMed
Summary

Protein-rich liquid-liquid phase separation (LLPS) can transition to solid aggregates, implicated in neurodegenerative diseases. This study reveals how hydration water balance dictates this transition, offering insights for protein design.

More Related Videos

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.1K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.7K

Related Experiment Videos

Last Updated: Jul 13, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.1K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.7K

Area of Science:

  • Biophysics
  • Structural Biology
  • Neuroscience

Background:

  • Liquid-liquid phase separation (LLPS) of protein-rich condensates is crucial for cellular function.
  • The transition from LLPS to solid aggregates is implicated in neurotoxic diseases.
  • Understanding the molecular drivers of this transition is vital for therapeutic development.

Purpose of the Study:

  • To investigate the role of hydration water in the transition from LLPS to liquid-solid phase separation (LSPS).
  • To observe LLPS and LSPS in real-time using a novel spectroscopic approach.
  • To elucidate the balance of protein-water interactions governing phase behavior.

Main Methods:

  • Utilized vibrational THz spectroscopy to probe hydration water dynamics.
  • Studied a medically relevant cellular prion protein.
  • Induced LLPS and LSPS through targeted protein mutations.

Main Results:

  • Identified a balance between hydrophobic and hydrophilic solvation contributions controlling phase separation.
  • Hydrophobic hydration drives phase separation via water release (entropic).
  • Hydrophilic hydration stabilizes liquid condensates (enthalpic), preventing aggregation.

Conclusions:

  • Modifying protein hydrophilicity influences the transition between LLPS and LSPS.
  • Reduced hydrophilicity promotes the shift from LLPS to LSPS.
  • This molecular understanding enables rational protein design for controlling phase behavior.