Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of vitamin D receptor and calcium sensing receptor in parathyroid cancer.

Brazilian journal of otorhinolaryngology·2026

Same author

Secreted frizzled-related protein 1 augments vascular smooth muscle cell calcification via activating the Wnt/β-catenin/Runx2 axis.

International urology and nephrology·2026

Same author

Response to "Comment on 'Unsupervised Machine Learning for Differential Analysis in Proteomics' ".

Analytical chemistry·2026

Same author

TensoGraph: a tensor-transformer framework for global-local drug synergy prediction on heterogeneous graphs.

Briefings in bioinformatics·2026

Same author

Minimally Invasive Single-Cell Metabolomics Analysis to Unveil Different Trajectories of Individual Cells in Ferroptosis.

Analytical chemistry·2026

Same author

Changes in frailty and all-cause mortality in patients on hemodialysis: a prospective cohort study.

BMC nephrology·2026

Search research articles

Related Experiment Video

Updated: Jul 13, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

Microfluidics Coupled Mass Spectrometry for Single Cell Multi-Omics.

Dongxue Zhang¹, Liang Qiao¹

¹Department of Chemistry, Institutes of Biomedical Sciences, and Minhang Hospital, Fudan University, Shanghai, 20000, China.

|October 16, 2023

Summary

This summary is machine-generated.

Single-cell analysis using microfluidics overcomes population-level limitations, revealing cellular heterogeneity. This approach enables deeper insights into cellular phenotypes and biological states through advanced omics techniques.

Keywords:

mass spectrometry microfluidics multi-omics single-cell

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Published on: December 22, 2017

Related Experiment Videos

Last Updated: Jul 13, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog Xenopus laevis Embryos

Published on: December 22, 2017

Area of Science:

Biotechnology
Cell Biology
Analytical Chemistry

Background:

Population-level analysis obscures critical cellular heterogeneity, limiting understanding of biological processes.
Microfluidics offers precise control over individual cells, enabling advanced single-cell analysis.
Single-cell omics provides deep molecular insights, differentiating cell types and states.

Purpose of the Study:

To review microfluidic chip designs for single-cell manipulation (isolation, trapping, sorting).
To summarize microfluidic applications in single-cell proteomics, metabolomics, lipidomics, and multi-omics.
To discuss current challenges and future prospects in single-cell multi-omics.

Main Methods:

Review of microfluidic chip designs: droplet, microwell, hydrodynamic, and microvalve-based systems.
Compilation of studies utilizing microfluidics coupled with mass spectrometry for single-cell omics.
Analysis of techniques for single-cell isolation, manipulation, trapping, screening, and sorting.

Main Results:

Microfluidic technologies effectively enable diverse single-cell analyses.
Integration with mass spectrometry allows for comprehensive single-cell omics profiling.
Various microfluidic platforms facilitate detailed molecular characterization at the single-cell level.

Conclusions:

Microfluidics is crucial for dissecting cellular heterogeneity and understanding biological complexity.
Single-cell multi-omics, powered by microfluidics, offers unprecedented biological insights.
Addressing challenges in single-cell multi-omics will unlock future applications in biology and medicine.