Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The distinct trimeric structure of the immunodominant chlamydial antigen Major Outer Membrane Protein.

Nature communications·2026
Same author

Protocol for Engineered Compositional Asymmetry Within Nanodiscs.

Membranes·2026
Same author

Novel polymer fixed-target microfluidic platforms with an ultra-thin moisture barrier for serial macromolecular crystallography.

bioRxiv : the preprint server for biology·2025
Same author

Planar, Kinked, or Twisted? Molecular Structures of Blue and Red Phase Polydiacetylene Langmuir Films.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Fluorogenic Biosensing with Tunable Polydiacetylene Vesicles.

Biosensors·2025
Same author

Cell-Free Screening, Production and Animal Testing of a STI-Related Chlamydial Major Outer Membrane Protein Supported in Nanolipoproteins.

Vaccines·2024

Related Experiment Video

Updated: Jul 13, 2025

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.8K

All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.

Kevin K Gu1, Zhongrui Liu1, Sankar Raju Narayanasamy2

  • 1Department of Chemical Engineering, University of California at Davis, Davis, California 95616, USA.

Biomicrofluidics
|October 16, 2023
PubMed
Summary

Fixed-target systems revolutionize serial crystallography by enabling efficient delivery of microcrystals to X-ray Free Electron Laser (XFEL) sources. These systems are crucial for determining challenging protein structures.

More Related Videos

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.4K
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

6.5K

Related Experiment Videos

Last Updated: Jul 13, 2025

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.8K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.4K
Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

6.5K

Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • X-ray Free Electron Laser (XFEL) sources and serial crystallography have advanced protein structure determination.
  • Efficient microcrystal delivery is essential for XFEL-based crystallography.
  • Handling microcrystals for XFEL experiments presents significant challenges.

Purpose of the Study:

  • To detail a fixed-target system for serial crystallography sample delivery.
  • To highlight the advantages and versatility of fixed-target systems.
  • To guide researchers in the application of fixed-target technology.

Main Methods:

  • Discussion of a fixed-target system for microcrystal encapsulation.
  • Description of rastering the fixed target through the X-ray beam.
  • Examination of microfluidic-based fixed-target innovations.

Main Results:

  • Fixed-target systems are user-friendly and maintain sample hydration.
  • These systems accommodate diverse sample types and beamline needs.
  • Microfluidic fixed targets show promise as a versatile tool for serial crystallography.

Conclusions:

  • Fixed-target systems represent a significant advancement in serial crystallography sample delivery.
  • Further development of microfluidic fixed targets is needed.
  • These systems are poised to become a "workhorse" for structural biology research.