Association between miR-365 polymorphism and ischemic stroke in a Chinese population

Area of Science:

• Genetics
• Molecular Biology
• Neurology

Background:

• Ischemic stroke (IS) is a leading cause of global mortality and morbidity.
• Aberrant microRNA-365 (miR-365) expression is implicated in diseases like atherosclerosis and cancer.
• Single-nucleotide polymorphisms (SNPs) in miRNA genes are crucial for understanding disease susceptibility.

Purpose of the Study:

• To investigate the association between miR-365 single-nucleotide polymorphisms (SNPs) and the risk of ischemic stroke (IS).
• To explore the relationship between miR-365 expression levels and specific miR-365 genotypes in IS patients.

Main Methods:

• Genotyping of three miR-365 polymorphic loci (rs121224, rs30230, rs178553) using SNPscans in 215 IS patients and 220 controls.
• Quantification of miR-365 relative expression in peripheral blood mononuclear cells via real-time quantitative PCR.
• Statistical analysis including adjusted odds ratios (OR) and haplotype analysis.

Main Results:

• The miR-365 rs30230 polymorphism showed a significant association with reduced IS risk (e.g., TC vs. CC: OR=0.55, P=0.022).
• Haplotype analysis indicated that the C-T-G haplotype was linked to a decreased risk of IS (OR=0.68, P=0.047).
• miR-365 expression was significantly higher in IS patients than controls (P < 0.001), with lower levels in rs30230 TC/TT genotypes (P < 0.001).

Conclusions:

• The miR-365 rs30230 polymorphism is associated with ischemic stroke susceptibility in the studied population.
• The rs30230 TC/TT genotype may act as a protective factor against ischemic stroke.
• Altered miR-365 expression levels correlate with specific genotypes and IS risk.