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Quantitative proteomic analysis and verification identify global protein profiling dynamics in pig during the estrous
Haiyun Xin1,2, Baohong Li1, Fanming Meng1,2
1State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Frontiers in Veterinary Science
|October 16, 2023
Summary
This study used mass spectrometry to analyze pig saliva during the estrous cycle, identifying key proteins. These findings offer potential for more accurate and efficient estrus detection methods in sows.
Area of Science:
- Proteomics
- Animal Reproduction
- Biochemistry
Background:
- Current estrus detection methods in pigs are inefficient and inaccurate.
- Understanding physiological changes during the estrous cycle is crucial for improving detection accuracy.
Purpose of the Study:
- To explore salivary proteome profiles throughout the estrous cycle in pigs.
- To identify potential protein biomarkers for improved estrus detection.
Main Methods:
- Label-free acquisition mass spectrometry was employed to analyze salivary proteomes at different stages of the estrous cycle.
- Parallel reaction monitoring (PRM) was used to verify the relative protein expression profiles.
Main Results:
- A total of 1,155 proteins were identified, with 115 differentially expressed proteins (DEPs) found among the groups.
- DEPs were functionally annotated to calcium ion binding, actin cytoskeleton, and lyase activity.
- PRM confirmed the expression consistency of specific proteins, including PHB domain-containing protein and carbonic anhydrase.
Conclusions:
- Salivary proteomic analysis provides valuable insights into the estrous cycle of sows.
- The identified DEPs and verified proteins represent potential biomarkers for developing novel estrus detection strategies.
