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Related Experiment Video

Updated: Jul 13, 2025

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Published on: April 1, 2009

Establishing Riboglow-FLIM to visualize noncoding RNAs inside live zebrafish embryos.

Nadia Sarfraz¹, Harrison J Lee¹, Morgan K Rice¹

¹Department of Chemistry, Georgetown University, Washington, District of Columbia.

Biophysical Reports

|October 16, 2023

Summary

This summary is machine-generated.

We developed Riboglow-FLIM, a novel RNA visualization platform for live zebrafish embryos. This method allows robust, quantitative detection of RNA in whole organisms, revealing cell-to-cell differences.

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Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish

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Published on: June 27, 2011

Area of Science:

Molecular Biology
Cell Biology
Developmental Biology

Background:

Accurate visualization of RNA in living systems is crucial for understanding cellular functions and disease mechanisms.
Live zebrafish embryos serve as a valuable model for studying multicellular complexity and disease.
Current RNA visualization techniques in whole organisms are limited, necessitating advanced imaging tools.

Purpose of the Study:

To establish and evaluate the Riboglow-FLIM platform for robust RNA visualization in live biological systems.
To adapt and optimize the Riboglow-FLIM platform for imaging RNA within whole zebrafish embryos.
To demonstrate the capability of Riboglow-FLIM in resolving cellular heterogeneity of RNA populations in vivo.

Main Methods:

Systematic evaluation of Fluorescence Lifetime Imaging Microscopy (FLIM) capabilities within the Riboglow platform.
Utilizing adherent mammalian cells for initial RNA visualization and optimization.
Injecting labeled mammalian cells into zebrafish embryos for cell-by-cell analysis in a whole-organism context.
Employing a far-red Cy5-based variant of the Riboglow platform for enhanced imaging in zebrafish.

Main Results:

Quantitative assessment of Riboglow-FLIM parameters confirmed its efficacy for RNA imaging.
Successful detection and robust quantification of a model noncoding RNA within live zebrafish embryos.
Demonstrated ability to resolve cell-to-cell heterogeneity in RNA populations using the developed platform.
Validation of the platform's applicability in a complex multicellular model.

Conclusions:

Riboglow-FLIM provides a powerful and quantitative method for visualizing RNA in live zebrafish embryos.
The platform overcomes limitations in current whole-organism RNA imaging techniques.
This technology holds significant promise for diverse research fields, including developmental biology and disease modeling.