Integrated analysis of NET-DNA receptor CCDC25 in malignant tumors: Pan-cancer analysis

Area of Science:

• Oncology
• Molecular Biology
• Immunology

Background:

• Coiled-coil domain containing 25 (CCDC25) is implicated in neutrophil extracellular trap (NET) formation.
• CCDC25's role in various cancers remains incompletely understood.

Purpose of the Study:

• To systematically analyze CCDC25 expression across 30 cancer types and acute myeloid leukemia.
• To investigate the correlation between CCDC25 expression, clinical outcomes, and the tumor microenvironment.

Main Methods:

• Utilized GTEx and CCLE databases for expression profiling in normal and cancer tissues.
• Analyzed TCGA pan-cancer data for survival analysis (Kaplan-Meier, COX regression).
• Assessed CCDC25's association with tumor microenvironment components, neoantigens, and genomic instability markers.

Main Results:

• CCDC25 shows broad expression in normal tissues, with moderate to low levels in cancer cell lines.
• No significant correlation was found between CCDC25 levels and overall/disease-free survival in the TCGA dataset.
• CCDC25 expression was associated with the infiltration of specific immune cells, including T cells, mast cells, dendritic cells, M2 macrophages, and neutrophils.

Conclusions:

• CCDC25 generally lacks prognostic value across most cancer types.
• CCDC25 presents a potential therapeutic target for managing cancer metastasis and immune infiltration.