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Updated: Jul 13, 2025
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
Published on: November 29, 2014
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Real-Time Detection of Dynamic Affinity between Biomolecules Using Surface Plasmon Resonance (SPR) Technology
Wenqiao An1, Zhihao Sun1, Pengmei Guo2
1School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine.
Journal of Visualized Experiments : Jove
|October 16, 2023
Summary
Surface Plasmon Resonance (SPR) technology offers sensitive, label-free detection for biomolecules, viruses, and food safety. This method enables rapid, real-time drug screening and pharmacokinetic analysis.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Biophysics
Background:
- Surface Plasmon Resonance (SPR) is a label-free optical detection method.
- SPR technology enables sensitive and precise biomolecular detection.
- Applications include virus detection, drug screening, and food safety analysis.
Purpose of the Study:
- To introduce the principles and applications of SPR technology.
- To detail the operational process of SPR for biomolecular analysis.
- To demonstrate SPR's utility in drug screening with specific binding examples.
Main Methods:
- Instrument calibration and system operation.
- Ligand immobilization and analyte binding analysis.
- Multi-cycle analysis and real-time kinetic data acquisition.
Main Results:
- Demonstrated SPR for label-free detection of biomolecular interactions.
- Successfully analyzed the binding of quercetin and calycosin to KCNJ2 protein in real-time.
- Obtained detailed binding kinetics and specificity data.
Conclusions:
- SPR technology is highly specific, simple, sensitive, and rapid.
- SPR is effective for high-throughput drug screening and pharmacokinetic studies.
- SPR is valuable for virus detection and environmental/food safety identification.
