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Related Concept Videos

Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
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Related Experiment Video

Updated: Jul 24, 2026

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration
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Temporal-spatial Generation of Astrocytes in the Developing Diencephalon.

Wentong Hong1, Pifang Gong1, Xinjie Pan1

  • 1Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Neuroscience Bulletin
|October 16, 2023
PubMed
Summary

Radial glia in the embryonic mouse brain

Keywords:
Astrocyte specificationDiencephalonLineage tracingRadial gliaThird ventricle

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Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Glial Cell Biology

Background:

  • Astrocytes are the most abundant glial cells in the mammalian brain, but their developmental origins and regional fate specification remain poorly understood.
  • Understanding astrocyte development is crucial for deciphering brain formation and function.

Purpose of the Study:

  • To investigate the lineage, fate specification, and migration patterns of astrocytes originating from the radial glia of the third ventricle (3V) wall in the embryonic mouse brain.
  • To identify region-specific gene expression patterns that may govern astrogenesis in the diencephalon.

Main Methods:

  • In-utero electroporation for lineage tracing of radial glia progenitors along the 3V wall.
  • Genetic fate mapping to track astrocyte populations.
  • Transcriptomic analysis of regional 3V wall and lateral ventricle (LV) wall samples.

Main Results:

  • Radial glia along the 3V wall exhibit regional differences, with upper regions generating astrocytes and lower regions generating neurons.
  • The first astrocytes in the diencephalon emerge early in the dorsal region, specifically along the zona incerta.
  • Transcriptomic analysis revealed distinct gene expression profiles in the dorsal 3V wall compared to ventral 3V and LV walls, potentially regulating dorsal astrogenesis.

Conclusions:

  • Astrocyte generation in the developing mouse diencephalon follows a distinct spatiotemporal pattern.
  • Regional differences in radial glia progenitors along the 3V wall dictate cell fate (astrocytes vs. neurons).
  • Specific gene expression in the dorsal 3V wall may be key drivers of dorsal astrogenesis.