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Updated: Jul 13, 2025

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
Published on: July 30, 2020
Following the Birth, Life, and Death of mRNAs in Single Cells
Bastian T Eichenberger1,2, Esther Griesbach1, Jessica Mitchell1
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland;
Abstract:
Recent advances in single-molecule imaging of mRNAs in fixed and living cells have enabled the lives of mRNAs to be studied with unprecedented spatial and temporal detail. These approaches have moved beyond simply being able to observe specific events and have begun to allow an understanding of how regulation is coupled between steps in the mRNA life cycle. Additionally, these methodologies are now being applied in multicellular systems and animals to provide more nuanced insights into the physiological regulation of RNA metabolism.
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