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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
Published on: January 12, 2015
Developmental expression of peroxiredoxin gene family in early embryonic development of Xenopus tropicalis
Linke Zhong1, Tingting Fu1, Chengdong Wang2
1Key Laboratory of Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, People's Republic of China; Joint Laboratory for Regenerative Medicine, Chinese University of Hong Kong-Jinan University, Guangzhou 510632, China; International Base of Collaboration for Science and Technology (JNU), Ministry of Science and Technology, Guangzhou 510632, Guangdong, China; Department of Developmental and Regenerative Biology, Jinan University, Guangzhou 510632, China.
Abstract:
Peroxidase genes (Prdx) encode a family of antioxidant proteins, which can protect cells from oxidative damage by reducing various cellular peroxides. This study investigated the spatiotemporal expression patterns of gene members in this family during the early development of Xenopus tropicalis. Real-time quantitative PCR showed that all members of this gene family have a distinct temporal expression pattern during the early development of X. tropicalis embryos. Additionally, whole mount in situ hybridization revealed that individual prdx genes display differential expression patterns, with overlapping expression in lymphatic vessels, pronephros, proximal tubule, and branchial arches. This study provides a basis for further study of the function of the prdx gene family.
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