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Updated: Jul 13, 2025
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms
Hosam M Al-Jehani1, Alaa N Turkistani1, Majd M Alrayes1
1From the Department of Neurosurgery (Al-Jehani), Interventional Neuroradiology and Critical Care Medicine, Department of Neurology (AlHamid, Alabbas), King Fahad Hospital of the University, Imam Abdulrahman bin Faisal University, AL Khobar, from the College of Medicine (Alrayes, Al Sadah), Imam Abdulrahman bin Faisal University, Department of Biochemistry (Cyrus, Al-Ali), College of Medicine, Imam Abdulrahman bin Faisal University, Dammam, and from the Department of Neurosurgery (Turkistani), King Faisal Specialist Hospital and Research Center, and from the Department of Neurosurgery (AlShamikh), National Neuroscience Institute, King Fahad Medical City Riyadh, Kingdom of Saudi Arabia.
Objectives:
To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.
Methods:
This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.
Results:
A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls (p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not.
Conclusion:
miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.
