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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

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One-step embedding method for maintaining orientation of pathological tissue specimens using agar thin films.

Atsushi Uchiyama^1,2, Fumikazu Kimura³, Yoshie Wakatsuki¹

¹Division of Diagnostic pathology, Tokyo Medical University Hachioji Medical Center, Hachioji, Japan.

Journal of Clinical Pathology

|October 16, 2023

Summary

Agar thin films (ATFs) maintain tissue fragment order and orientation during histological processing. This method also reduces tissue embedding time, making it ideal for busy pathology labs.

Keywords:

Diagnostic Techniques and Procedures Laboratory Manual Medical Laboratory Science Pathology, Surgical Quality Assurance, Health Care

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Area of Science:

Histopathology
Laboratory Medicine
Biomedical Engineering

Background:

Accurate pathological diagnosis relies on maintaining tissue fragment order and orientation during histological processing.
Standard tissue embedding procedures can be time-consuming and may risk dislodging or misorienting specimens.

Purpose of the Study:

To present and evaluate a novel method using agar thin films (ATFs) to maintain tissue fragment order and orientation during histological embedding.
To assess the impact of ATFs on the efficiency of the tissue embedding process.

Main Methods:

Agar thin films (ATFs) were integrated with tissue samples within standard histology cassettes.
Embedding time was compared between cassettes with and without ATFs using porcine liver tissue.
The procedure was performed by medical laboratory scientists with varying experience levels.

Main Results:

Integration of ATFs enabled one-step tissue sample embedding.
A significant average reduction of 6.22 seconds in embedding time per cassette was observed when using ATFs compared to traditional methods.
ATFs demonstrated ease of preparation and long-term storage in formalin, allowing for immediate use.

Conclusions:

Agar thin films effectively maintain tissue fragment order and orientation during histological embedding.
The use of ATFs streamlines the embedding process, reducing overall procedure time.
This technique offers a practical and efficient solution for busy pathology laboratories seeking to improve workflow and diagnostic accuracy.