An all-in-one portable colorimetric detection platform for sensitive detection of bisphenol A based on...

BPA aptamers functionalized cerium oxide nanoparticles encapsulated in zeolitic imidazolate framework-8 (CeO₂@ZIF-8/Apt) were developed to fabricate an all-in-one portable platform for on-site quantitative detection of BPA. By combining biocomposites with a 3,3',5,5'-tetramethylbenzidine (TMB)-based sodium alginate (SA) hydrogel and smartphone-based RGB analysis, highly sensitive and convenient monitoring of BPA was achieved. CeO₂@ZIF-8 composites were constructed using a novel surfactant-modified concentration-controlled synthesis strategy. After being functionalized with BPA aptamers, CeO₂@ZIF-8/Apt biocomposites were used as target-response colorimetric probes for target recognition and signal transduction. The oxidase-like activity of CeO₂@ZIF-8 was effectively sealed by BPA aptamers and controllably released in a concentration-dependent manner through aptamer-BPA reactions. Utilizing SA hydrogels containing TMB in the caps, a one-step sample addition and one-pot detection can be conveniently achieved and reliably quantified by smartphone-based RGB analysis in an instrument-free way. The detection range of this portable detection platform is 50 pg/mL to 500 ng/mL with limit of detection calculated as 34.88 pg/mL, comparable to that of conventional detection in the solution system (4.57 pg/mL). The recoveries in tap water, apple juice, and milk ranged from 91.02 % and 106.75 %. This work contributes new insights into the design of all-in-one detection platforms for contaminants monitoring in resource-constrained regions.