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Updated: Jul 13, 2025
Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation
Published on: August 26, 2014
Follow the time course of inflammation caused by intraperitoneal administration of multi-wall carbon nanotubes in
Masanori Horie1, Sakiko Sugino1, Tomoki Ohno1
1Health and medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.
Objective:
Understand the progress of inflammation over time caused by multi-walled carbon nanotubes (MWCNT).
Methods:
Two types of MWCNTs were administered to C57BL/6N mice via intraperitoneal administration at low and high doses (0.05 and 1.0 mg/mouse, respectively). Inflammation was evaluated until 6 months after administration based on cytokine levels and pathological observations. The abdominal cavity lavage fluid was collected and analyzed 1 week, 1, 3, and 6 month(s) after administration. IL-6 expression markedly increased 3 months after the administration of high-dose MWCNT-7.
Results:
Notable inflammation was observed in the groups administered with one of the MWCNT, MWCNT-7. On the other hand, inflammation in another MWCNT-treated group was milder than that in the MWCNT-7-treated group. MWCNT-7 induced pronounced inflammation but did not induce tumor formation during the experimental period. Inflammation reaction is one of the most important biological responses to MWCNT.
Conclusion:
Three months post-exposure becomes a turning point for the harmful effects of the intraperitoneally administered MWCNT-7.
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