Related Experiment Video
Updated: Jul 13, 2025
10:18
Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo
Published on: September 11, 2018
5.9K
Cellular Interactions in Cell Sheets Enhance Mesenchymal Stromal Cell Immunomodulatory Properties
Celia M Dunn1,2, Sumako Kameishi1, Tavie Parker1,2
1Cell Sheet Tissue Engineering Center (CSTEC), Department of Molecular Pharmaceutics, University of Utah, Salt Lake City, Utah, USA.
Tissue Engineering. Part A
|October 17, 2023
Summary
Cell sheet technology enhances mesenchymal stromal cells (MSCs) for therapy by increasing immunomodulatory factors and improving engraftment. This scaffold-free method boosts therapeutic potential through enhanced cell-cell interactions.
Area of Science:
- Cellular Biology
- Tissue Engineering
- Immunology
Background:
- Mesenchymal stromal cells (MSCs) are used in cell therapy for their immunomodulatory properties to reduce inflammation.
- Patient outcome variability in MSC therapy is linked to poor engraftment and retention of heterogeneous cells.
- Developing strategies to improve MSC engraftment and therapeutic efficacy is crucial.
Purpose of the Study:
- To investigate the potential of human single-cell-derived clonal bone marrow MSC (hcBMSC) sheets produced via cell sheet technology for enhanced immunomodulatory function.
- To evaluate the impact of cell sheet fabrication on the expression of key immunomodulatory molecules.
- To explore the role of intercellular interactions in cell sheet-based MSC therapy.
Main Methods:
- Fabrication of scaffold-free hcBMSC sheets using cell sheet technology.
- Comparison of immunomodulatory molecule expression (IL-10, IDO-1, PTGES2) in cell sheets versus conventional cell suspensions.
- Assessment of the effect of gap junction inhibition (carbenoxolone) on gene expression in cell sheets.
Main Results:
- Cell sheet fabrication significantly upregulated immunomodulatory molecules (IL-10, IDO-1, PTGES2) in GMP-grade hcBMSC lines and whole MSCs compared to cell suspensions.
- Treatment with a gap junction inhibitor reduced IL-10 and IDO-1 expression in cell sheets, indicating the importance of intercellular communication.
- Cell sheet technology facilitates the production of directly transferable multicellular constructs with amplified therapeutic factor expression.
Conclusions:
- Cell sheet technology is a viable strategy to enhance the immunomodulatory capacity of MSCs for therapeutic applications.
- Intercellular interactions within cell sheets play a critical role in maintaining and amplifying MSC therapeutic functions.
- This approach offers a promising method to overcome variability issues and improve the consistency of MSC-based therapies.
More Related Videos
Related Concept Videos
01:19Mesenchymal Stem Cells
4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
01:20Cell Adhesion Molecules - Types and Functions
6.8K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
6.8K
01:24Overview of Cell-Matrix Interactions
7.3K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.3K
01:26Stem Cell Niche
5.1K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.1K
01:29The Extracellular Matrix
9.0K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
9.0K
01:26Extracellular Matrix
2.9K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
2.9K