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Related Experiment Video

Updated: Jul 13, 2025

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Scanning single molecule localization microscopy (scanSMLM) for super-resolution volume imaging.

Jigmi Basumatary¹, Neptune Baro¹, Prakash Joshi¹

¹Nanobioimaging Laboratory, Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore, 560012, India.

Communications Biology

|October 17, 2023

Summary

This summary is machine-generated.

We developed scanSMLM, a rapid 3D imaging system using single-molecule localization microscopy. This technique visualizes nanoscale biological structures and protein clustering, offering new insights into diseases like Influenza-A.

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Last Updated: Jul 13, 2025

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Published on: June 8, 2020

Area of Science:

Biophysics
Optical Microscopy
Cell Biology

Background:

Single-molecule localization microscopy (SMLM) offers nanoscale resolution beyond diffraction limits.
Existing SMLM techniques have limitations in rapid 3D volume imaging.

Purpose of the Study:

Introduce a novel scanning SMLM (scanSMLM) system for rapid 3D volume imaging.
Enhance visualization of nanoscale biological structures and molecular distributions in 3D.

Main Methods:

Implemented a scanning-based 4f detection system with an electrically-tunable lens (ETL) for axial plane selection.
Utilized cyclic and conventional scanning schemes for volume acquisition.
Applied scanSMLM to image actin filaments, mitochondrial proteins, and influenza HA protein clusters.

Main Results:

Achieved rapid 3D volume imaging with SMLM.
Successfully visualized actin filament architecture and mitochondrial protein distribution.
Enabled the first 3D visualization of Hemagglutinin (HA) protein clusters in transfected cells, revealing their distribution across the entire cell volume.

Conclusions:

The scanSMLM system provides a powerful tool for high-resolution 3D imaging of biological systems.
Offers unprecedented insights into the biophysical parameters of protein clustering in disease progression, exemplified by Influenza-A.
Facilitates a deeper understanding of molecular organization within cells at the nanoscale.