Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary Cells.

Micromachines·2026
Same author

Dynamic changes in mRNA isoform usage during human retinal development.

bioRxiv : the preprint server for biology·2025
Same author

Myeloid lineage C3 induces reactive gliosis and neuronal stress during CNS inflammation.

Nature communications·2025
Same author

A novel approach to increase glial cell populations in brain microphysiological systems.

bioRxiv : the preprint server for biology·2023
Same author

Helminth egg derivatives as proregenerative immunotherapies.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

Ex Vivo Integration of Human Stem Retinal Ganglion Cells into the Mouse Retina.

Cells·2022
Same journal

Significance of Reynolds Number Consistency in Non-Newtonian Hemodynamic Simulations: Insights from Fontan Circulation.

Physics of fluids (Woodbury, N.Y. : 1994)·2026
Same journal

Spatiotemporal distribution of the intraglottal pressure and vocal fold contact pressure in excised larynges.

Physics of fluids (Woodbury, N.Y. : 1994)·2025
Same journal

<i>In vitro</i> characterization of solute transport in the spinal canal.

Physics of fluids (Woodbury, N.Y. : 1994)·2025
Same journal

Evaluating the accuracy of one-dimensional glottal flow model in predicting voice production: comparison to experiments and three-dimensional flow simulations.

Physics of fluids (Woodbury, N.Y. : 1994)·2025
Same journal

Semi-analytical solutions of passive scalar transport in generalized Newtonian fluid flow.

Physics of fluids (Woodbury, N.Y. : 1994)·2025
Same journal

Time-dependent diffusion in one-dimensional disordered media decorated by permeable membranes: Theoretical findings backed by simulations and a new disorder class.

Physics of fluids (Woodbury, N.Y. : 1994)·2025
See all related articles

Related Experiment Video

Updated: Jul 13, 2025

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

2.7K

Deciphering viscoelastic cell manipulation in rectangular microchannels.

Takayuki Suzuki1, Srivathsan Kalyan1, Cynthia Berlinicke2

  • 1Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Physics of Fluids (Woodbury, N.Y. : 1994)
|October 18, 2023
PubMed
Summary
This summary is machine-generated.

Viscoelastic focusing in rectangular microchannels is explored to understand cell manipulation. This study provides design insights for improved flow cytometry and particle sorting.

More Related Videos

A Microfluidic Technique to Probe Cell Deformability
09:47

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

11.3K
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
10:31

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

Published on: September 27, 2012

20.0K

Related Experiment Videos

Last Updated: Jul 13, 2025

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
05:49

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

2.7K
A Microfluidic Technique to Probe Cell Deformability
09:47

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

11.3K
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
10:31

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

Published on: September 27, 2012

20.0K

Area of Science:

  • Biophysics
  • Microfluidics
  • Fluid Dynamics

Background:

  • Viscoelastic focusing offers label-free manipulation of micro/nanoscale bioparticles.
  • Understanding flow conditions is crucial for designing effective viscoelastic focusing microfluidic devices.
  • Viscoelastic focusing in straight, rectangular microchannels remains incompletely understood.

Purpose of the Study:

  • Investigate inertial, elastic, and viscoelastic focusing of biological cells in rectangular microchannels.
  • Elucidate the mechanisms governing cell focusing by systematically varying fluid elasticity and inertia.
  • Characterize hydrodynamic focusing behavior based on cell size, flow rate, and fluid properties.

Main Methods:

  • Cells were injected into microchannels with a fixed, non-unity aspect ratio.
  • Images were captured from two orientations to determine cross-sectional equilibrium positions from 2D projections.
  • Systematic variation of fluid elasticity and inertia to study cell focusing.

Main Results:

  • Hydrodynamic focusing behaviors were characterized concerning cell size, flow rate, and fluid characteristics.
  • Insights into flow characteristics influencing equilibrium positions were obtained.
  • Demonstrated potential for enhancing detection accuracy in flow cytometry and sorting resolution in particle sorting.

Conclusions:

  • This work advances the understanding of viscoelastic focusing in rectangular microchannels.
  • Provides valuable design guidelines for microfluidic devices utilizing viscoelastic focusing.
  • Contributes to the advancement of viscoelastic particle manipulation techniques and their applications.