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Comparative Metabolomic Profiles of Vascular Involvement in Behçet's Disease
Çiğdem Yücel1, Erdim Sertoğlu1, Ahmet Omma2
1Department of Clinical Biochemistry, University of Health Sciences, Gülhane Training and Research Hospital, Ankara, Turkey.
Insights
Metabolomic analysis reveals that Behçet's disease with vascular involvement is linked to altered amino acid and oxidative stress pathways, particularly glutathione synthesis. These findings offer new insights into disease pathogenesis and potential diagnostic markers.
Area of Science:
- Biochemistry
- Immunology
- Genetics
Background:
- Behçet's disease is a systemic inflammatory condition with significant vascular complications.
- Vascular involvement is a primary driver of morbidity and mortality in Behçet's disease.
- Limited understanding exists regarding the pathogenesis and vascular incidence in Behçet's disease.
Purpose of the Study:
- To investigate the distinct metabolic profiles of Behçet's disease patients experiencing vascular involvement.
- To identify unique metabolic signatures associated with vascular complications in Behçet's disease.
Main Methods:
- Serum samples from 48 Behçet's disease patients (18 with vascular involvement) and 40 healthy controls were analyzed.
- Untargeted metabolomics was performed using gas chromatography-mass spectrometry.
- Multivariate and univariate statistical analyses were employed to identify altered metabolites and pathways.
Main Results:
- A total of 168 metabolites were identified across the study groups.
- Significant alterations in amino acid metabolism and oxidative stress pathways, including glutathione synthesis, were observed in Behçet's disease patients.
- These metabolic changes were particularly pronounced in patients with vascular involvement.
Conclusions:
- Untargeted metabolomics provides novel molecular insights into Behçet's disease pathogenesis and vascular involvement.
- Vascular complications in Behçet's disease are strongly associated with disruptions in amino acid metabolism and the antioxidant system.
- Identified metabolic pathways warrant further investigation for potential diagnostic and prognostic applications in Behçet's disease and its vascular manifestations.
Background:
Behçet's disease is a systemic, inflammatory disease affecting multiple organs. Vascular involvement is the main cause of morbidity and mortality in Behçet's disease patients. Though clinically well-defined, there is limited information related to disease pathogenesis and vascular incidence in this patient group. The aim of this study is to investigate the unique metabolic signatures of Behçet's disease patients with vascular involvement.
Methods:
Metabolomic profiling was performed on serum samples of 48 Behçet's disease patients (18 with vascular involvement) and 40 healthy controls using gas chromatography-mass spectrometrybased untargeted metabolomics analysis. Multivariate and univariate statistical analyses were performed to find altered metabolites and pathways.
Results:
Untargeted metabolomics results showed that a total of 168 metabolites were identified. The comparison between the groups of Behçet's disease, vascular involvement in Behçet's disease, and the healthy control group showed that altered amino acid and oxidative stress pathways, especially with glutathione synthesis, could be an important stage for developing Behçet's disease.
Conclusion:
In the present work, the untargeted metabolomics approach provided new molecular insights for a better understanding of Behçet's disease pathogenesis and also developing vascular involvement in Behçet's disease at the metabolite level. The results showed that vascular involvement in Behçet's disease could be highly linked with amino acid metabolism and also the antioxidant system, and these disease-related pathways could be evaluated with further experiments for diagnosis and prognosis of Behçet's disease and also for vascular involvement in Behçet's disease.
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