Sinomonas cellulolyticus sp. nov., isolated from Loktak lake

A novel cellulolytic strain JC656^T was isolated from the rhizosphere soil of Alisma plantago-aquatica of floating island (Phumdis) of Loktak lake, Manipur, India. The 16S rRNA gene sequence similarities between strain JC656^T and other Sinomonas type strains ranged between 98.5 and 97.3%, wherein strain JC656^T exhibited the highest sequence similarity (98.5%) to Sinomonas notoginsengisoli KCTC 29237^T. Colonies were yellow-colored and grew aerobically. Cells were gram-positive, rod-shaped and non-motile. The optimal growth of the strain JC656^T occured at 28 °C and pH 7. Strain JC656^T contained MK-9 as the predominant isoprenoid quinone and anteiso-C_15:0, iso-C_16:0 and anteiso-C_17:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and a glycolipid were the polar lipids. Strain JC656^T contained lysine, alanine, glutamine, diaminopimelic acid (DAP) and two unidentified amino acids as characteristic cell wall amino acids. The genome size of strain JC656^T was 3.9 Mb with a DNA G + C content of 69.9 mol %. For the affirmation of the strain's taxonomic status, a detailed phylogenomic study was done. Based on its phylogenetic position and morphological, physiological, and genomic features, strain JC656^T represents a new species of the genus Sinomonas, for which we propose the name Sinomonas cellulolyticus sp. nov. The type strain JC656^T = (KCTC 49339^T = NBRC 114142^T).