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Related Concept Videos

Photoluminescence: Applications01:14

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Jul 13, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Pyrene-acylhydrazone-based Turn-on Fluorescent Probe for Highly Sensitive Detection Cu2+ and Application in

Lu Liu1, Hanshu Zhang1, Yun Gao1

  • 1Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education; Yunnan Provincial Center for Research & Development of Natural Products; School of Pharmacy, Yunnan University, Kunming, 650500, P. R. China.

Journal of Fluorescence
|October 18, 2023
PubMed
Summary

A new fluorescent probe detects copper ions (Cu2+) with high sensitivity and selectivity. This biocompatible probe shows low toxicity and is effective for imaging copper in live cells.

Keywords:
BioimagingCopper ionPyrene-acylhydrazoneTurn-on fluorescent probe

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Developing selective fluorescent probes for copper ions (Cu2+) is crucial for environmental and life science applications.
  • Existing probes often lack the desired sensitivity, selectivity, or biocompatibility.
  • Copper ions play vital roles in biological processes, but their dysregulation is linked to various diseases.

Purpose of the Study:

  • To synthesize and characterize a novel "turn-on" fluorescent probe for sensitive and selective detection of Cu2+.
  • To investigate the sensing mechanism and binding properties of the probe with Cu2+.
  • To evaluate the probe's applicability for intracellular imaging of Cu2+ in live cells.

Main Methods:

  • One-step condensation reaction for probe synthesis.
  • Characterization using 1H NMR, 13C NMR, and HRMS.
  • Fluorescence spectroscopy for selectivity and detection limit determination.
  • Job's plot analysis and HRMS for binding stoichiometry.
  • 1H NMR titration and FT-IR for binding model investigation.
  • Cellular toxicity assays and live-cell imaging.

Main Results:

  • A novel pyrene-acylhydrazone-based fluorescent probe (T) was successfully synthesized.
  • Probe T demonstrated high selectivity and a low detection limit (0.304 nM) for Cu2+.
  • The sensing mechanism involves dual PET-TICT pathways with a 1:1 binding stoichiometry.
  • Probe T exhibited low cytotoxicity and good membrane permeability.
  • Successful application in fluorescent imaging of intracellular Cu2+ in HT-22 cells.

Conclusions:

  • The developed probe T is a highly selective and sensitive tool for Cu2+ detection.
  • The probe's biocompatibility and cell-imaging capabilities make it valuable for biological studies.
  • This work offers a promising platform for developing advanced fluorescent probes for metal ion sensing.