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LuMA-Functionalized Thermosensitive Hydrogel: A Versatile and Robust Dopamine-Triggered Platform for Diverse
Yuanyuan Wang1,2, Xianwen Kan1
1Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Laboratory of Molecule-Based Materials, Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
ACS Applied Bio Materials
|October 18, 2023
Summary
A novel luminescence thermosensitive hydrogel enables sensitive detection of multiple biomarkers. This electrochemiluminescence-resonance energy transfer (ECL-RET) system enhances signal amplification for early disease diagnosis and monitoring.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Biotechnology
Background:
- Single biomarkers are insufficient for accurate early diagnosis, disease monitoring, and prognosis.
- Developing sensitive and reliable methods for multiple biomarker analysis is crucial for clinical applications.
Purpose of the Study:
- To synthesize a luminescence thermosensitive hydrogel for sensitive electrochemiluminescence-based detection of multiple biomarkers.
- To investigate the mechanism of signal quenching and amplification via resonance energy transfer (RET) and thermosensitive properties.
Main Methods:
- Synthesized a hydrogel using luminol (LuMA), N-isopropylacrylamide (NIPAM), and DNA aptamers for dopamine (DA) detection.
- Utilized electrochemiluminescence (ECL) and resonance energy transfer (RET) for DA detection, leveraging temperature-induced hydrogel collapse for signal amplification.
- Applied the hydrogel system with a duplex-specific nuclease (DSN) strategy for multiplexed detection of miRNA-141 and MUC1.
Main Results:
- Achieved sensitive detection of DA with a limit of detection (LOD) of 1.7 × 10⁻¹⁰ M.
- Demonstrated 1.4 times signal amplification due to temperature-induced hydrogel collapse enhancing RET efficiency.
- Successfully detected multiple targets, including miRNA-141 (LOD 2.5 aM) and MUC1 (LOD 1.6 fg/mL), showcasing the system's versatility.
Conclusions:
- The developed ECL-RET hydrogel is a versatile and robust platform for sensitive multiplexed biomarker detection.
- This approach holds significant potential for clinical diagnosis and disease therapeutic assays.
