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Multiple DNA repair pathways contribute to MMS-induced post-replicative DNA synthesis in S. pombe

Seong Min Kim1, Susan L Forsburg2

  • 1Molecular and Computational Biology, University of Southern California, Los Angeles, California, United States.

Micropublication Biology
|October 19, 2023
PubMed

Insights

DNA synthesis can occur outside of normal S-phase due to replication stress. Fission yeast utilize DNA repair pathways like translesion synthesis and break-induced replication for this post-replicative DNA synthesis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Replication stress can trigger DNA synthesis outside of the canonical S-phase.
  • Previous work showed fission yeast initiate G2-phase DNA synthesis in response to MMS, but not M-phase.

Purpose of the Study:

  • To investigate the DNA repair pathways involved in post-replicative DNA synthesis in fission yeast.
  • To identify key proteins and enzymes contributing to DNA synthesis outside of S-phase.

Main Methods:

  • Utilized fission yeast models.
  • Investigated the roles of various DNA repair pathways, including translesion synthesis and break-induced replication.
  • Examined the involvement of checkpoint kinases, repair proteins, and polymerases.

Main Results:

  • Confirmed that various DNA repair pathways contribute to post-replicative DNA synthesis.
  • Demonstrated the involvement of translesion synthesis and break-induced replication.
  • Identified checkpoint kinases, repair/resection proteins, and multiple polymerases as crucial components.

Conclusions:

  • Fission yeast employ diverse DNA repair mechanisms to achieve DNA synthesis outside of S-phase.
  • The process is complex, involving multiple proteins and pathways to maintain genome integrity under stress.

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