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Updated: Jul 12, 2025
A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
A novel approach for 25-gauge transconjunctival sutureless vitrectomy to evaluate vitreous substitutes in rabbits
Rui-Jin Ran1,2, Ting Wang1, Meng-Ying Tao1
1Department of Ophthalmology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China.
Aim:
To improve the standard three-port vitrectomy for establishing and evaluating an endotamponade model in rabbits.
Methods:
Three ports were prepared near the third eyelid of rabbits, and the infusion port was placed at the inferior nasal quadrant with the inserted cannula linking with a self-designed handheld rigid infusion catheter. All right eyes of rabbits underwent a modified 25-gauge vitrectomy and were subsequently filled with balanced salt solution, silicone oil, and eight-arm polyethylene glycols (8-arm PEGs) hydrogel separately for comparison. Ophthalmic examinations were performed regularly to record the changes after the surgery.
Results:
Successful vitrectomy was achieved among 44 chinchilla rabbits. The mean operation time was 4.51±1.25min. Four eyes (9.1%) presented limited lens touch and two eyes (4.5%) showed retinal touch during surgery. Incision leakage was found in three eyes (6.8%) after surgery. There was no endophthalmitis, hemorrhage, or retinal detachment during the observation period and ophthalmic examinations after the implantation of vitreous substitutes.
Conclusion:
The modified technique of the standard vitrectomy applied in the endotamponade model in rabbits shows excellent safety and practicality.
