Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Curvilinear Motion: Rectangular Components

Curvilinear Motion: Rectangular Components

Curvilinear motion characterizes the movement of a particle or object along a curved path, notably evident when envisioning a car navigating a winding road. If the car starts at point A, its position vector is established within a fixed frame of reference, where the ratio of the position vector to its magnitude signifies the unit vector pointing in the position vector's direction.
As the car advances, its position evolves over time. Quantifying the car's velocity involves computing the...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms.

Npj imaging·2026

Same author

AI caption generation model for digital pathology of adenocarcinoma in endoscopic histopathology using multi-instance attention mechanisms.

Scientific reports·2026

Same author

Super-resolution functional photoacoustic microscopy via label-free cell tracking.

Light, science & applications·2026

Same author

UV curable resin as a rapid and superior sealant for STORM.

Biomedical optics express·2025

Same author

Giant single-step upconversion via sub-35-fs phonon dynamics in the nonlinear optical regime.

Science advances·2025

Same author

Yes-Associated Protein (YAP) Is Associated With Disease Severity and Visual Prognosis in Epiretinal Membrane.

Investigative ophthalmology & visual science·2025

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 12, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

Enhancing obSTORM imaging performance with cubic spline PSF modeling.

Donghoon Koo¹, Minchol Lee¹, Youngseop Lee¹

¹Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea.

Biomedical Optics Express

|October 19, 2023

Summary

This summary is machine-generated.

A new cubic spline model improves oblique plane super-resolution microscopy (obSTORM) for thick biological samples. This enhanced point spread function (PSF) model increases imaging resolution and axial range, enabling faster 3D imaging of tissues.

More Related Videos

Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

Published on: May 10, 2020

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Related Experiment Videos

Last Updated: Jul 12, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

Published on: May 10, 2020

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Area of Science:

Biophysics
Optical Microscopy
Super-resolution Imaging

Background:

Oblique plane microscopy (obSTORM) offers super-resolution imaging for thick biological specimens.
Existing Gaussian point spread function (PSF) models limit obSTORM resolution and axial localization range due to poor PSF shape fitting.
This restricts the application of obSTORM in imaging complex biological structures.

Purpose of the Study:

To develop a more accurate point spread function (PSF) model for obSTORM.
To enhance 3D localization precision and improve volumetric imaging capabilities.
To overcome the limitations of Gaussian PSF models in obSTORM.

Main Methods:

Implementation of cubic splines for precise modeling of experimental PSF shapes in obSTORM.
Application of the refined PSF model to super-resolution imaging of mouse retina tissues.
Quantitative analysis of imaging resolution, localization precision, and axial range improvements.

Main Results:

The cubic spline PSF model significantly enhanced 3D localization precision compared to Gaussian models.
Imaging resolution in mouse retina tissues increased by approximately 1.2 times.
Seamless stitching of single molecules and a doubling of the sectional interval were achieved in volumetric obSTORM.

Conclusions:

Cubic spline PSF modeling provides a superior approach for accurate and efficient obSTORM imaging.
This advancement enables higher resolution and faster volumetric imaging of biological specimens.
The refined model extends the utility of obSTORM for studying thick tissues and small animals.