Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Stomach Histology

Stomach Histology

The stomach comprises several layers that work together to facilitate digestion and protect the organ. The outermost layer is called the serosa, which provides support and protection to the stomach. The muscularis externa layer is responsible for the mechanical breakdown of food by contracting and moving the stomach. The submucosa layer, located beneath the muscularis externa, contains connective tissue, blood vessels, nerves, and glands that secrete mucus and other substances essential for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.

Scientific reports·2026

Same author

Characterization of inner ear granular bodies by polarization-resolved second harmonic generation microscopy.

Biomedical optics express·2026

Same author

Erratum: Imaging otoconia by second harmonic generation microscopy: erratum.

Biomedical optics express·2026

Same author

Polar organization of H&E dyes in histology tissue revealed by polarimetric nonlinear microscopy.

Biomedical optics express·2025

Same author

Second harmonic generation of myofibrils exhibits a polarization-resolved "gradient" effect.

Biomedical optics express·2025

Same author

Third-order nonlinear optical properties of a series of pyrroles and dipyrroles featuring π-conjugated substituents and linkers.

Physical chemistry chemical physics : PCCP·2025

Search research articles

Related Experiment Video

Updated: Jul 12, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy.

Hwanhee Jeon¹, MacAulay Harvey¹, Richard Cisek¹

¹Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia, B3H 3C3, Canada.

Biomedical Optics Express

|October 19, 2023

Summary

Polarization-resolved second harmonic generation (PSHG) microscopy reveals significant collagen ultrastructure changes in gastric adenocarcinoma. This technique may enable automated cancer diagnosis by detecting collagen disorder.

More Related Videos

Author Spotlight: Gastric Epithelial Cell Responses in Helicobacter pylori infection

Author Spotlight: Gastric Epithelial Cell Responses in Helicobacter pylori infection

Published on: July 5, 2024

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Published on: March 24, 2023

Related Experiment Videos

Last Updated: Jul 12, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Author Spotlight: Gastric Epithelial Cell Responses in Helicobacter pylori infection

Author Spotlight: Gastric Epithelial Cell Responses in Helicobacter pylori infection

Published on: July 5, 2024

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Published on: March 24, 2023

Area of Science:

Biomedical Optics
Materials Science
Oncology

Background:

Collagen's ultrastructure is critical in tissue biomechanics and disease.
Gastric adenocarcinoma involves alterations in the tumor microenvironment, including extracellular matrix changes.

Purpose of the Study:

To investigate collagen ultrastructural differences between normal gastric tissue and gastric adenocarcinoma.
To evaluate the potential of polarization-resolved second harmonic generation (PSHG) microscopy for quantitative analysis and cancer diagnosis.

Main Methods:

Utilized polarization-resolved second harmonic generation (PSHG) microscopy to image collagen.
Applied cylindrical and trigonal symmetry assumptions to extract quantitative PSHG parameters (ρ, κ, S).
Performed numerical simulations of crossing fibrils to interpret PSHG parameter variations.

Main Results:

Observed statistically significant variations in PSHG parameters (ρ, κ, S) in gastric adenocarcinoma compared to normal tissue.
Indicated increased collagen disorder in gastric adenocarcinoma.
Correlated higher S parameter values with increased intersecting collagen fibrils of varying diameters.
Demonstrated the ability of PSHG parameters to differentiate between various grades of gastric adenocarcinoma.

Conclusions:

PSHG microscopy effectively quantifies collagen ultrastructural alterations in gastric adenocarcinoma.
The observed collagen disorder is a key differentiator between cancerous and normal gastric tissue.
PSHG-based quantitative parameters show promise for the automated diagnosis and grading of gastric cancer.