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Related Experiment Video

Updated: Jul 12, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Blood component separation in straight microfluidic channels.

Lap Man Lee¹, Ketan H Bhatt¹, Dustin W Haithcock¹

¹CFD Research Corporation, Huntsville, Alabama 35806, USA.

Biomicrofluidics

|October 19, 2023

Summary

This summary is machine-generated.

This study presents a novel microfluidic device for rapid blood component separation without dilution or lysis. The passive system achieves high purity for platelets and efficient red blood cell removal, enhancing field diagnostics.

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Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

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Related Experiment Videos

Last Updated: Jul 12, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

Published on: June 4, 2015

Area of Science:

Biomedical Engineering
Microfluidics
Hematology

Background:

Traditional blood component separation relies on labor-intensive centrifugation.
Field and resource-limited blood processing requires advanced, portable technologies.
Existing microfluidic methods often necessitate sample pretreatment or exhibit lower efficiency.

Purpose of the Study:

To develop a rapid, passive microfluidic device for efficient blood component separation.
To improve separation efficiency using integrated fluidic restrictors.
To demonstrate versatile operation modes for various blood fractionation applications.

Main Methods:

Utilized a small footprint, passive microfluidic channel device.
Leveraged margination and inertial focusing effects for separation.
Incorporated fluidic restrictors at outlet ports to enhance separation precision.
Employed straight microfluidic channels with a high aspect ratio rectangular cross section.

Main Results:

Achieved 95.4% platelet purity from human whole blood.
Demonstrated 99.9% red blood cell (RBC) removal rate during plasma extraction.
Successfully concentrated platelet-rich plasma by 2.6×.
Showcased scalable, continuous, and clog-free operation.

Conclusions:

The developed microfluidic device offers a versatile and efficient solution for blood component separation.
This technology bypasses the need for sample dilution, lysis, or labeling, preserving sample integrity.
The system is suitable for integration into multi-step workflows for advanced sample preparation and diagnostics.