Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

392
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
392
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2025 OIC Manufacturing "Road Runner" Challenge [Invited].

Applied optics·2026
Same author

Aluminum-Silica Core-Shell Nanoparticles via Nonthermal Plasma Synthesis.

Nanomaterials (Basel, Switzerland)·2025
Same author

Capacitively coupled nonthermal plasma synthesis of aluminum nanocrystals for enhanced yield and size control.

Nanotechnology·2023
Same author

2022 Optical Interference Coatings Conference: Manufacturing Problem Contest [Invited].

Applied optics·2023
Same author

Comparison of a Fully Weight-Based Protocol with a Non-Weight-Based Dosage Titration Protocol for IV Unfractionated Heparin: A Before-and-After Study.

The Canadian journal of hospital pharmacy·2023
Same author

Modeling fluorescence reemission in the one-dimensional radiative transfer problem using the P3 approximation.

Journal of the Optical Society of America. A, Optics, image science, and vision·2021

Related Experiment Video

Updated: Jul 12, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

13.5K

Integrating sphere port error in diffuse reflectance measurements.

Luke J Sandilands, Thomas Cameron

    Applied Optics
    |October 19, 2023
    PubMed
    Summary

    Finite integrating sphere port thickness causes light loss, underestimating diffuse reflectance measurements. This study quantifies the error using Monte Carlo simulations and experiments, offering strategies to minimize this measurement inaccuracy.

    Area of Science:

    • Optical Metrology
    • Spectrophotometry
    • Radiometry

    Background:

    • Integrating spheres are crucial for diffuse reflectance measurements.
    • Finite port thickness can introduce systematic errors in optical measurements.
    • Accurate diffuse reflectance is vital in material science and colorimetry.

    Purpose of the Study:

    • To investigate the impact of finite integrating sphere port thickness on diffuse reflectance measurements.
    • To quantify the light loss and resulting measurement error.
    • To explore factors influencing this error and propose mitigation strategies.

    Main Methods:

    • Combined numerical (Monte Carlo ray tracing) and experimental approach.
    • Utilized integrating sphere reflectometers with varying port geometries.

    More Related Videos

    Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
    04:54

    Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

    Published on: June 16, 2023

    3.0K
    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    12.5K

    Related Experiment Videos

    Last Updated: Jul 12, 2025

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.5K
    Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging
    04:54

    Author Spotlight: A Stable Phantom Material for Optical and Acoustic Imaging

    Published on: June 16, 2023

    3.0K
    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    12.5K
  • Analyzed effects of sample reflectance, port dimensions, and illumination size.
  • Main Results:

    • Finite port thickness leads to underestimation of diffuse reflectance due to scattering losses.
    • Monte Carlo simulations accurately predicted measurement errors.
    • Experimental data validated the numerical findings across different port geometries.

    Conclusions:

    • Finite integrating sphere port thickness is a significant, often overlooked, source of measurement error.
    • Understanding and quantifying these losses is essential for accurate diffuse reflectance determination.
    • Strategies exist to minimize this error in integrating sphere designs and usage.